Development and Optimization of an UPLC-QTOF-MSMS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species
Joint Authors
Steenkamp, P. A.
Madala, N. E.
Tugizimana, F.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chlorogenic acids (CGA) have been profiled in the leaves of Momordica balsamina, Momordica charantia, and Momordica foetida.
All three species were found to contain the trans and cis isomers of 4-acyl para-coumaroylquinic acid (pCoQA), caffeoylquinic acid (CQA), and feruloylquinic acid (FQA).
To the best of our knowledge, this is the first report of pCoQA and FQA and their cis isomers in these Momordica species.
These profiles were obtained by a newly developed UPLC-qTOF-MS method based on the in-source collision induced dissociation (ISCID) method optimized to mimic the MS2 and MS3 fragmentation of an ion trap-based MS.
The presence of the cis isomers is believed to be due to high UV exposure of these plants.
Furthermore, the absence of the 3-acyl and 5-acyl CGA molecules points to a metabolic mark that is unusual and represents a very interesting biochemical phenotype of these species.
Our optimized ISCID method was also shown to be able to distinguish between the geometrical isomers of all three forms of CGA, a phenomenon previously deemed impossible with other common mass spectrometry systems used for CGA analyses.
American Psychological Association (APA)
Madala, N. E.& Tugizimana, F.& Steenkamp, P. A.. 2014. Development and Optimization of an UPLC-QTOF-MSMS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1039835
Modern Language Association (MLA)
Madala, N. E.…[et al.]. Development and Optimization of an UPLC-QTOF-MSMS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1039835
American Medical Association (AMA)
Madala, N. E.& Tugizimana, F.& Steenkamp, P. A.. Development and Optimization of an UPLC-QTOF-MSMS Method Based on an In-Source Collision Induced Dissociation Approach for Comprehensive Discrimination of Chlorogenic Acids Isomers from Momordica Plant Species. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1039835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1039835