Pharmacokinetic Comparison of Isoalantolactone and Alantolactone in Rats after Administration Separately by Optimization of an UPLC-MS2 Method
Joint Authors
Wang, Mengyue
Li, Xiaobo
Peng, Ying
Xu, Renjie
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Isoalantolactone and alantolactone are two major active ingredients that are present in many medicinal plants.
In this study, a sensitive and rapid ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of the two compounds in rat plasma, separately.
In this method, an electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) was selected for quantification using target fragment ions 233.2→187.1 for isoalantolactone (alantolactone) and 245.1→189.1 for internal standard (IS).
Retention time of the lactones and IS was within 3.0 min.
Further calibration suggested a linear regression can be calculated within 2.5–500 ng/mL for isoalantolactone and 4–500 ng/mL for alantolactone.
This method was used to compare the pharmacokinetic characteristics of isoalantolactone and alantolactone at a single dose of 5 mg/kg into male Sprague-Dawley rats by intravenous administration separately.
The levels of t1/2, Kel, CL, Cmax, and AUC were significantly increased in the alantolactone group compared to isoalantolactone.
These results suggested that isoalantolactone was distributed and eliminated more rapidly than alantolactone in rats when administered, respectively.
American Psychological Association (APA)
Xu, Renjie& Wang, Mengyue& Peng, Ying& Li, Xiaobo. 2014. Pharmacokinetic Comparison of Isoalantolactone and Alantolactone in Rats after Administration Separately by Optimization of an UPLC-MS2 Method. Journal of Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1039957
Modern Language Association (MLA)
Xu, Renjie…[et al.]. Pharmacokinetic Comparison of Isoalantolactone and Alantolactone in Rats after Administration Separately by Optimization of an UPLC-MS2 Method. Journal of Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1039957
American Medical Association (AMA)
Xu, Renjie& Wang, Mengyue& Peng, Ying& Li, Xiaobo. Pharmacokinetic Comparison of Isoalantolactone and Alantolactone in Rats after Administration Separately by Optimization of an UPLC-MS2 Method. Journal of Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1039957
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1039957