Purification and Characterization of Bioactive Compounds from Styela clava
Joint Authors
Ju, Bao
Chen, Bin
Zhang, Xingrong
Han, Chunling
Jiang, Aili
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The immunomodulatory activity of extract from Styela clava was studied systematically based on activity tracking in vitro in order to find out novel-structured secondary metabolite.
The proliferation rates of mouse splenic lymphocytes and peritoneal macrophages were used as screening index, as well as NO release promoting activities.
The crude extract (CE) and its different polar fractions from S.
clava all exhibited proliferative activity of splenolymphocytes and mouse macrophages, as well as NO release promoting activities, among which petroleum ether fraction (PE) showed the strongest effect.
The antioxidant experiment in vitro showed that CE demonstrated antioxidant ability in 1,1-diphenyl-2-picrylhydrazyl (DPPH) system and the beta carotene-linoleic acid system; the activity of ethyl acetate fraction (ET) was much stronger than that of the others.
Further isolated by silica gel column chromatography, ET was classified into seven sub-components (E1~E7) listed in the order of activity as E 5 > E 6 > E 4 > E 3 > E 7 > E 2 > E 1 .
Five compounds were separated as (1) cholesteric-7-en-3β-ol, (2) cholesteric-4-en-3β,6β-diol, (3) cholesterol, (4) batilol, and (5) ceramide, among which (1), (2), and (4) were isolated for the first time from S.
clava.
American Psychological Association (APA)
Ju, Bao& Chen, Bin& Zhang, Xingrong& Han, Chunling& Jiang, Aili. 2014. Purification and Characterization of Bioactive Compounds from Styela clava. Journal of Chemistry،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040005
Modern Language Association (MLA)
Ju, Bao…[et al.]. Purification and Characterization of Bioactive Compounds from Styela clava. Journal of Chemistry No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040005
American Medical Association (AMA)
Ju, Bao& Chen, Bin& Zhang, Xingrong& Han, Chunling& Jiang, Aili. Purification and Characterization of Bioactive Compounds from Styela clava. Journal of Chemistry. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040005
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040005