Construction of Differential-Methylation Subtractive Library
Joint Authors
Hu, Wei
Liang, Xiaolei
Dong, Tian
Hu, Yanfeng
Liu, Jie
Mao, Lina
Liu, Xu
Bi, Yurong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Stress-induced ROS changes DNA methylation patterns.
A protocol combining methylation-sensitive restriction endonuclease (MS-RE) digestion with suppression subtractive hybridization (SSH) to construct the differential-methylation subtractive library was developed for finding genes regulated by methylation mechanism under cold stress.
The total efficiency of target fragment detection was 74.64%.
DNA methylation analysis demonstrated the methylation status of target fragments changed after low temperature or DNA methyltransferase inhibitor treatment.
Transcription level analysis indicated that demethylation of DNA promotes gene expression level.
The results proved that our protocol was reliable and efficient to obtain gene fragments in differential-methylation status.
American Psychological Association (APA)
Hu, Wei& Liang, Xiaolei& Dong, Tian& Hu, Yanfeng& Liu, Jie& Mao, Lina…[et al.]. 2014. Construction of Differential-Methylation Subtractive Library. Journal of Chemistry،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040010
Modern Language Association (MLA)
Hu, Wei…[et al.]. Construction of Differential-Methylation Subtractive Library. Journal of Chemistry No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1040010
American Medical Association (AMA)
Hu, Wei& Liang, Xiaolei& Dong, Tian& Hu, Yanfeng& Liu, Jie& Mao, Lina…[et al.]. Construction of Differential-Methylation Subtractive Library. Journal of Chemistry. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040010
