Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method
Joint Authors
Guo, Leilei
Tian, Xiangqin
Shan, Shangran
Han, Jian
Shang, Xiaojun
Ma, Suying
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A RP-HPLC-UV method was developed and validated for simultaneous determination of florfenicol and diclazuril in compound powder.
The separation involved using a SinoChoom ODS-BP C18 (5 μm, 4.6 mm × 250 mm) analytical column.
The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid (pH was adjusted to 3.0 with triethylamine).
The ratio of acetonitrile and 0.2% phosphoric acid in the mobile phase was 60 : 40 (v/v) from 0 minutes to 6 minutes and 70 : 30 (v/v) from 6.1 minutes to 15 minutes.
The flow rate was 1 mL/min.
The temperature of the analytical column was maintained at 30°C.
The detection was monitored at 225 nm and 277 nm for florfenicol and diclazuril, respectively.
The excipients in the compound powder did not interfere with the drug peaks.
The calibration curves of florfenicol and diclazuril were fairly linear over the concentration ranges between 50.0–500.0 μg/mL ( r = 0.9995 ) and 10.0–100.0 μg/mL ( r = 0.9992 ), respectively.
The RSD of both the intraday and interday variations was below 2.1% for florfenicol and diclazuril.
The method was successfully validated according to International Conference on Harmonisation and proved to be suitable for the simultaneous determination of florfenicol and diclazuril in compound powder.
American Psychological Association (APA)
Guo, Leilei& Tian, Xiangqin& Shan, Shangran& Han, Jian& Shang, Xiaojun& Ma, Suying. 2014. Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method. Journal of Chemistry،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040021
Modern Language Association (MLA)
Guo, Leilei…[et al.]. Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method. Journal of Chemistry No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1040021
American Medical Association (AMA)
Guo, Leilei& Tian, Xiangqin& Shan, Shangran& Han, Jian& Shang, Xiaojun& Ma, Suying. Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method. Journal of Chemistry. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040021
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040021