Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells
Joint Authors
Li, Gang
Zhang, Xiao-xiao
Lin, Lin
Liu, Xiao-ning
Ma, Cheng-jun
Li, Ji
Wang, Chi-bo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of this study is to evaluate the protection of ginsenoside Rg3 against oxidative stress in human neuroblastoma SK-N-SH cells.
20(R)-ginsenoside Rg3 (20(R)-Rg3) and 20(S)-ginsenoside Rg3 (20(S)-Rg3) were prepared by the method of chemical degradation and column chromatography, and the structure of the two compounds was characterized by 1H-NMR and 13C-NMR spectroscopy.
MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h).
We also investigated the changes of intracellular MDA content, SOD activity, and ROS formation after the treatment of ginsenoside Rg3 for 20 h.
The results indicated that both 20 (R)-Rg3 and 20 (S)-Rg3 had obvious protection against H2O2-induced oxidative stress in SK-N-SH cells.
Moreover, 20(R)-Rg3 exhibited better antioxidant activity than 20(S)-Rg3 in vitro.
These findings are expected to provide some implication for further research and application of ginsenoside Rg3 in neuroprotection.
American Psychological Association (APA)
Li, Gang& Zhang, Xiao-xiao& Lin, Lin& Liu, Xiao-ning& Ma, Cheng-jun& Li, Ji…[et al.]. 2014. Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells. Journal of Chemistry،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1040102
Modern Language Association (MLA)
Li, Gang…[et al.]. Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells. Journal of Chemistry No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1040102
American Medical Association (AMA)
Li, Gang& Zhang, Xiao-xiao& Lin, Lin& Liu, Xiao-ning& Ma, Cheng-jun& Li, Ji…[et al.]. Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells. Journal of Chemistry. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1040102
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040102