Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells

Joint Authors

Zhou, Jieqiong
Li, Weihua
Xie, Qiang
Hou, Yuxi
Zhan, Shaopeng
Yang, Xi
Xu, Xiaofeng
Cai, Jun
Huang, Zhengrong

Source

Journal of Diabetes Research

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-06-04

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Diseases
Medicine

Abstract EN

The aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism.

MIN6 cells were, respectively, treated with 0 μM, 2 μM, 5 μM, and 10 μM simvastatin for 48 h.

Radio immunoassay was performed to measure the effect of simvastatin on insulin secretion in MIN6 cells.

Luciferase method was used to examine the content of ATP in MIN6 cells.

Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 (Cav1.2), and glucose transporter-2 (GLUT2), respectively.

ATP-sensitive potassium current and L-type calcium current were recorded by whole-cell patch-clamp technique.

The results showed that high concentrations of simvastatin (5 μM and 10 μM) significantly reduced the synthesis and secretion of insulin compared to control groups in MIN6 cells (P<0.05).

ATP content in simvastatin-treated cells was lower than in control cells (P<0.05).

Compared with control group, the mRNA and protein expression of Kir6.2 increased with treatment of simvastatin (P<0.05), and mRNA and protein expression of Cav1.2 and GLUT2 decreased in response to simvastatin (P<0.05).

Moreover, simvastatin increased the ATP-sensitive potassium current and reduced the L-type calcium current.

These results suggest that simvastatin inhibits the synthesis and secretion of insulin through a reduction in saccharometabolism in MIN6 cells.

American Psychological Association (APA)

Zhou, Jieqiong& Li, Weihua& Xie, Qiang& Hou, Yuxi& Zhan, Shaopeng& Yang, Xi…[et al.]. 2014. Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040291

Modern Language Association (MLA)

Zhou, Jieqiong…[et al.]. Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells. Journal of Diabetes Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040291

American Medical Association (AMA)

Zhou, Jieqiong& Li, Weihua& Xie, Qiang& Hou, Yuxi& Zhan, Shaopeng& Yang, Xi…[et al.]. Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040291

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1040291