The Protective Effects of Insulin and Natural Honey against Hippocampal Cell Death in Streptozotocin-Induced Diabetic Rats
Joint Authors
Jafari Anarkooli, Iraj
Barzegar Ganji, Hossein
Pourheidar, Maryam
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We investigated the effects of insulin and honey as antioxidants to prevent the hippocampal cell death in streptozotocin-induced diabetic rats.
We selected sixty Wister rats (5 groups of 12 animals each), including the control group (C), and four diabetic groups (control (D) and 3 groups treated with insulin (I), honey (H), and insulin plus honey (I + H)).
Diabetes was induced by streptozotocin injection (IP, 60 mg/kg).
Six weeks after the induction of diabetes, the group I received insulin (3-4 U/kg/day, SC), group H received honey (5 mg/kg/day, IP), and group I + H received a combination of the above at the same dose.
Groups C and D received normal saline.
Two weeks after treatment, rats were sacrificed and the hippocampus was extracted.
Neuronal cell death in the hippocampal region was examined using trypan blue assay, “H & E” staining, and TUNEL assay.
Cell viability assessment showed significantly lower number of living cells in group D than in group C.
Besides, the mean number of living cells was significantly higher in group I, H, and I + H compared to group D.
Therefore, it can be concluded that the treatment of the diabetic rats with insulin, honey, and a combination of insulin and honey can prevent neuronal cell death in different hippocampal areas of the studied samples.
American Psychological Association (APA)
Jafari Anarkooli, Iraj& Barzegar Ganji, Hossein& Pourheidar, Maryam. 2014. The Protective Effects of Insulin and Natural Honey against Hippocampal Cell Death in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040302
Modern Language Association (MLA)
Jafari Anarkooli, Iraj…[et al.]. The Protective Effects of Insulin and Natural Honey against Hippocampal Cell Death in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1040302
American Medical Association (AMA)
Jafari Anarkooli, Iraj& Barzegar Ganji, Hossein& Pourheidar, Maryam. The Protective Effects of Insulin and Natural Honey against Hippocampal Cell Death in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040302