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Reprogramming of Mice Primary Hepatocytes into Insulin-Producing Cells by Transfection with Multicistronic Vectors
Joint Authors
Luo, Haizhao
Chen, Rongping
Yang, Rui
Liu, Yan
Chen, Youping
Shu, Yi
Chen, Hong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The neogenesis of insulin-producing cells (IPCs) from non-beta-cells has emerged as a potential method for treating diabetes mellitus (DM).
Many groups have documented that activation of pancreatic transcription factor(s) in hepatocytes can improve the hyperglycemia in diabetic mice.
In the present study, we explored a novel protocol that reprogrammed primary hepatocytes into functional IPCs by using multicistronic vectors carrying pancreatic and duodenal homeobox-1 (Pdx1), neurogenin 3 (Ngn3), and v-musculoaponeurotic fibrosarcoma oncogene homolog A (MafA).
These triple-transfected cells activated multiple beta-cell genes, synthesized and stored considerable amounts of insulin, and released the hormone in a glucose-regulated manner in vitro.
Furthermore, when transplanted into streptozotocin-induced diabetic mice, the cells markedly ameliorated glucose tolerance.
Our results indicated that ectopic expression of Pdx1, Ngn3, and MafA facilitated hepatocytes-to-IPCs reprogramming.
This approach may offer opportunities for treatment of DM.
American Psychological Association (APA)
Luo, Haizhao& Chen, Rongping& Yang, Rui& Liu, Yan& Chen, Youping& Shu, Yi…[et al.]. 2014. Reprogramming of Mice Primary Hepatocytes into Insulin-Producing Cells by Transfection with Multicistronic Vectors. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040341
Modern Language Association (MLA)
Luo, Haizhao…[et al.]. Reprogramming of Mice Primary Hepatocytes into Insulin-Producing Cells by Transfection with Multicistronic Vectors. Journal of Diabetes Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040341
American Medical Association (AMA)
Luo, Haizhao& Chen, Rongping& Yang, Rui& Liu, Yan& Chen, Youping& Shu, Yi…[et al.]. Reprogramming of Mice Primary Hepatocytes into Insulin-Producing Cells by Transfection with Multicistronic Vectors. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040341