Ethnic Differences in MicroRNA-375 Expression Level and DNA Methylation Status in Type 2 Diabetes of Han and Kazak Populations
Joint Authors
Chang, Xiangyun
Li, Siyuan
Li, Jun
Yin, Liang
Zhou, Ting
Zhang, Chen
Chen, Xuan
Sun, Kan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Han population is six times as likely as Kazak population to present with type 2 diabetes mellitus (T2DM) in China.
We hypothesize that differential expression and CpG methylation of miR-375 may be an ethnic-related factor that influences the incidence of T2DM.
The expression level of miR-375 was examined using real-time PCR on Kazak and Han T2DM plasma samples.
Furthermore, the methylation levels of CpG sites of miR-375 promoter were determined by MassARRAY Spectrometry in these samples.
The relative expression levels of plasma miR-375 in Kazak T2DM samples are 1, and the relative expression levels of plasma miR-375 in Han T2DM samples are 3.
The mean level of miR-375 methylation, calculated from the methylation levels of the CpG sites, was 8.47% for the Kazak T2DM group and 10.38% for the Han T2DM group.
Further, five CpG units showed a statistically significant difference between Kazak and Han T2DM samples, and, among them, four were hypomethylated and only one CpG unit showed hypermethylation in Kazak T2DM samples.
These findings indicate that the expression levels of plasma miR-375 and its CpG methylation in the promoter region are ethnically different, which may contribute to the different incidence of diabetes observed in Kazak and Han populations.
American Psychological Association (APA)
Chang, Xiangyun& Li, Siyuan& Li, Jun& Yin, Liang& Zhou, Ting& Zhang, Chen…[et al.]. 2014. Ethnic Differences in MicroRNA-375 Expression Level and DNA Methylation Status in Type 2 Diabetes of Han and Kazak Populations. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040361
Modern Language Association (MLA)
Chang, Xiangyun…[et al.]. Ethnic Differences in MicroRNA-375 Expression Level and DNA Methylation Status in Type 2 Diabetes of Han and Kazak Populations. Journal of Diabetes Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040361
American Medical Association (AMA)
Chang, Xiangyun& Li, Siyuan& Li, Jun& Yin, Liang& Zhou, Ting& Zhang, Chen…[et al.]. Ethnic Differences in MicroRNA-375 Expression Level and DNA Methylation Status in Type 2 Diabetes of Han and Kazak Populations. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040361
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040361