Insulin Increases Ceramide Synthesis in Skeletal Muscle
Joint Authors
Hansen, M. E.
Tippetts, T. S.
Anderson, M. C.
Holub, Z. E.
Moulton, E. R.
Swensen, A. C.
Prince, J. T.
Bikman, B. T.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aims.
The purpose of this study was to determine the effect of insulin on ceramide metabolism in skeletal muscle.
Methods.
Skeletal muscle cells were treated with insulin with or without palmitate for various time periods.
Lipids (ceramides and TAG) were isolated and gene expression of multiple biosynthetic enzymes were quantified.
Additionally, adult male mice received daily insulin injections for 14 days, followed by muscle ceramide analysis.
Results.
In muscle cells, insulin elicited an increase in ceramides comparable to palmitate alone.
This is likely partly due to an insulin-induced increase in expression of multiple enzymes, particularly SPT2, which, when knocked down, prevented the increase in ceramides.
In mice, 14 days of insulin injection resulted in increased soleus ceramides, but not TAG.
However, insulin injections did significantly increase hepatic TAG compared with vehicle-injected animals.
Conclusions.
This study suggests that insulin elicits an anabolic effect on sphingolipid metabolism in skeletal muscle, resulting in increased ceramide accumulation.
These findings reveal a potential mechanism of the deleterious consequences of the hyperinsulinemia that accompanies insulin resistance and suggest a possible novel therapeutic target to mitigate its effects.
American Psychological Association (APA)
Hansen, M. E.& Tippetts, T. S.& Anderson, M. C.& Holub, Z. E.& Moulton, E. R.& Swensen, A. C.…[et al.]. 2014. Insulin Increases Ceramide Synthesis in Skeletal Muscle. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040364
Modern Language Association (MLA)
Hansen, M. E.…[et al.]. Insulin Increases Ceramide Synthesis in Skeletal Muscle. Journal of Diabetes Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040364
American Medical Association (AMA)
Hansen, M. E.& Tippetts, T. S.& Anderson, M. C.& Holub, Z. E.& Moulton, E. R.& Swensen, A. C.…[et al.]. Insulin Increases Ceramide Synthesis in Skeletal Muscle. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040364