Antiphospholipase A2 Receptor Autoantibodies: A Comparison of Three Different Immunoassays for the Diagnosis of Idiopathic Membranous Nephropathy
Joint Authors
Schiffer, Mario
Fritzler, Marvin J.
Behnert, Astrid
Müller-Deile, Janina
Beck, Laurence H.
Mahler, Michael
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-09
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
The recent identification of circulating autoantibodies directed towards the M-type phospholipase A2 receptor (PLA2R) has been a major advancement in the serological diagnosis of idiopathic membranous nephropathy (IMN), a common cause of nephrotic syndrome in adults.
The goal of this study was to compare the performance characteristics of two commercial assays as well as the first addressable laser bead immunoassay (ALBIA) developed for the detection of anti-PLA2R antibodies.
Methods.
Serum samples of 157 IMN patients and 142 controls were studied.
Samples were tested by a cell based immunofluorescence assay (CBA-IFA, Euroimmun, Germany), by ELISA (Euroimmun), and by a novel ALBIA employing an in vivo expressed recombinant human PLA2R.
Results.
Overall, the three assays showed significant qualitative and quantitative correlation.
As revealed by receiver operating characteristic analysis, the ALBIA correlated better with the CBA-IFA than the ELISA (P=0.0003).
The clinical sensitivities/specificities for IMN were 60.0% (51.0–68.5%)/98.6% (95.0–99.8%) and 56.2% (47.2–64.8%)/100.0% (97.4–100.0%) for ALBIA and CBA-IFA, respectively.
Conclusion.
The ALBIA represents a promising assay for the detection of anti-PLA2R antibodies showing similar performance to the CBA-IFA and the advantage of ease of use and suitability for high throughput, rapid turnaround times, and multiplexing.
American Psychological Association (APA)
Behnert, Astrid& Schiffer, Mario& Müller-Deile, Janina& Beck, Laurence H.& Mahler, Michael& Fritzler, Marvin J.. 2014. Antiphospholipase A2 Receptor Autoantibodies: A Comparison of Three Different Immunoassays for the Diagnosis of Idiopathic Membranous Nephropathy. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040770
Modern Language Association (MLA)
Behnert, Astrid…[et al.]. Antiphospholipase A2 Receptor Autoantibodies: A Comparison of Three Different Immunoassays for the Diagnosis of Idiopathic Membranous Nephropathy. Journal of Immunology Research No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1040770
American Medical Association (AMA)
Behnert, Astrid& Schiffer, Mario& Müller-Deile, Janina& Beck, Laurence H.& Mahler, Michael& Fritzler, Marvin J.. Antiphospholipase A2 Receptor Autoantibodies: A Comparison of Three Different Immunoassays for the Diagnosis of Idiopathic Membranous Nephropathy. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040770