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Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy
Joint Authors
Copple, Susan S.
Jaskowski, Troy D.
Giles, Rashelle
Hill, Harry R.
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To evaluate NOVA View with focus on reading archived images versus microscope based manual interpretation of ANA HEp-2 slides by an experienced, certified medical technologist.
Methods.
369 well defined sera from: 44 rheumatoid arthritis, 50 systemic lupus erythematosus, 35 scleroderma, 19 Sjögren’s syndrome, and 10 polymyositis patients as well as 99 healthy controls were examined.
In addition, 12 defined sera from the Centers for Disease Control and 100 random patient sera sent to ARUP Laboratories for ANA HEp-2 IIF testing were included.
Samples were read using the archived images on NOVA View and compared to results obtained from manual reading.
Results.
At a 1 : 40/1 : 80 dilution the resulting comparison demonstrated 94.8%/92.9% positive, 97.4%/97.4% negative, and 96.5%/96.2% total agreements between manual IIF and NOVA View archived images.
Agreement of identifiable patterns between methods was 97%, with PCNA and mixed patterns undetermined.
Conclusion.
Excellent agreements were obtained between reading archived images on NOVA View and manually on a fluorescent microscope.
In addition, workflow benefits were observed which need to be analyzed in future studies.
American Psychological Association (APA)
Copple, Susan S.& Jaskowski, Troy D.& Giles, Rashelle& Hill, Harry R.. 2014. Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040772
Modern Language Association (MLA)
Copple, Susan S.…[et al.]. Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy. Journal of Immunology Research No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1040772
American Medical Association (AMA)
Copple, Susan S.& Jaskowski, Troy D.& Giles, Rashelle& Hill, Harry R.. Interpretation of ANA Indirect Immunofluorescence Test Outside the Darkroom Using NOVA View Compared to Manual Microscopy. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1040772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040772