A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies
Joint Authors
Petrovski, Goran
Veréb, Zoltán
Andjelic, Sofija
Facskó, Andrea
Hawlina, Marko
Lumi, Xhevat
Josifovska, Natasha
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies.
Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study.
We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells.
Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.
American Psychological Association (APA)
Andjelic, Sofija& Lumi, Xhevat& Veréb, Zoltán& Josifovska, Natasha& Facskó, Andrea& Hawlina, Marko…[et al.]. 2014. A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040794
Modern Language Association (MLA)
Andjelic, Sofija…[et al.]. A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. Journal of Immunology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040794
American Medical Association (AMA)
Andjelic, Sofija& Lumi, Xhevat& Veréb, Zoltán& Josifovska, Natasha& Facskó, Andrea& Hawlina, Marko…[et al.]. A Simple Method for Establishing Adherent Ex Vivo Explant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040794