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Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA PrimingIntramuscular Recombinant Vaccinia Boosting Regimen
Joint Authors
Qiu, Sugan
Ben, Yinyin
Ren, Yanqin
Zhang, Xiaoyan
Wan, Yanmin
Wang, Jing
Ren, Xiaonan
Xu, Jianqing
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-01
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Previous study showed that CTB (Cholera toxin subunit B) can be used as a genetic adjuvant to enhance the systemic immune responses.
To further investigate whether it can also be used as a genetic adjuvant to improve mucosal immune responses, we constructed DNA and recombinant Tiantan vaccinia (rTTV) vaccines expressing OVA-CTB fusion antigen.
Female C57BL/6 mice were immunized with an intranasal DNA priming/intramuscular rTTV boosting regimen.
OVA specific T-cell responses were measured by IFN-γ ELISPOT and specific antibody responses were determined by ELISA.
Compared to the nonadjuvant group (pSV-OVA intranasal priming/rTTV-OVA intramuscular boosting), pSV-OVA-CTB intranasal priming/rTTV-OVA-CTB intramuscular boosting group significantly improved the magnitudes of T-cell responses at spleen (1562±567 SFCs/106 splenocytes versus 330±182 SFCs/106 splenocytes, P<0.01), mesenteric LN (96±83 SFCs/106 lymphocytes versus 1±2 SFCs/106 lymphocytes, P<0.05), draining LNs of respiratory tract (109±60 SFCs/106 lymphocytes versus 2±2 SFCs/106 lymphocytes, P<0.01) and female genital tract (89±48 SFCs/106 lymphocytes versus 23±21 SFCs/106 lymphocytes, P<0.01).
These results collectively demonstrated that fusion-expressed CTB could act as a potent adjuvant to improve both systemic and mucosal T-cell responses.
American Psychological Association (APA)
Qiu, Sugan& Ren, Xiaonan& Ben, Yinyin& Ren, Yanqin& Wang, Jing& Zhang, Xiaoyan…[et al.]. 2014. Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA PrimingIntramuscular Recombinant Vaccinia Boosting Regimen. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1040833
Modern Language Association (MLA)
Qiu, Sugan…[et al.]. Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA PrimingIntramuscular Recombinant Vaccinia Boosting Regimen. Journal of Immunology Research No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1040833
American Medical Association (AMA)
Qiu, Sugan& Ren, Xiaonan& Ben, Yinyin& Ren, Yanqin& Wang, Jing& Zhang, Xiaoyan…[et al.]. Fusion-Expressed CTB Improves Both Systemic and Mucosal T-Cell Responses Elicited by an Intranasal DNA PrimingIntramuscular Recombinant Vaccinia Boosting Regimen. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1040833
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040833