Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Joint Authors
Dankmeyer, Jennifer L.
Fast, Randy L.
Cote, Christopher K.
Worsham, Patricia L.
Fritz, David
Fisher, Diana
Kern, Steven J.
Merkel, Tod
Kirschning, Carsten J.
Amemiya, Kei
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The current candidate vaccine against Yersinia pestis infection consists of two subunit proteins: the capsule protein or F1 protein and the low calcium response V protein or V-antigen.
Little is known of the recognition of the vaccine by the host’s innate immune system and how it affects the acquired immune response to the vaccine.
Thus, we vaccinated Toll-like receptor (Tlr) 2, 4, and 2/4-double deficient, as well as signal adaptor protein Myd88-deficient mice.
We found that Tlr4 and Myd88 appeared to be required for an optimal immune response to the F1-V vaccine but not Tlr2 when compared to wild-type mice.
However, there was a difference between the requirement for Tlr4 and MyD88 in vaccinated animals.
When F1-V vaccinated Tlr4 mutant (lipopolysaccharide tolerant) and Myd88-deficient mice were challenged by aerosol with Y.
pestis CO92, all but one Tlr4 mutant mice survived the challenge, but no vaccinated Myd88-deficient mice survived the challenge.
Spleens from these latter nonsurviving mice showed that Y.
pestis was not cleared from the infected mice.
Our results suggest that MyD88 appears to be important for both an optimal immune response to F1-V and in protection against a lethal challenge of Y.
pestis CO92 in F1-V vaccinated mice.
American Psychological Association (APA)
Dankmeyer, Jennifer L.& Fast, Randy L.& Cote, Christopher K.& Worsham, Patricia L.& Fritz, David& Fisher, Diana…[et al.]. 2014. Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1040844
Modern Language Association (MLA)
Dankmeyer, Jennifer L.…[et al.]. Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice. Journal of Immunology Research No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1040844
American Medical Association (AMA)
Dankmeyer, Jennifer L.& Fast, Randy L.& Cote, Christopher K.& Worsham, Patricia L.& Fritz, David& Fisher, Diana…[et al.]. Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1040844
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040844