Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway
Joint Authors
Li, Weijuan
Li, Zhi
Chen, Yaoqi
Li, Songhai
Lv, Yuanyuan
Zhou, Wenping
Liao, Mengyang
Zhu, Feng
Zhou, Zihua
Cheng, Xiang
Zeng, Qiutang
Liao, Yuhua
Wei, Yumiao
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Our study intended to prove whether agonistic autoantibodies to angiotensin II type 1 receptor (AT1-AAs) exist in patients with coronary heart disease (CHD) and affect the human endothelial cell (HEC) by upregulating proinflammatory cytokines expression involved in NF-κB pathway.
Antibodies were determined by chronotropic responses of cultured neonatal rat cardiomyocytes coupled with receptor-specific antagonists (valsartan and AT1-EC2) as described previously.
Interleukin-6 (IL-6), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic protein-1 (MCP-1) expression were improved at both mRNA and protein levels in HEC, while NF-κB in the DNA level was improved detected by electrophoretic mobility shift assays (EMSA).
These improvements could be inhibited by specific AT1 receptor blocker valsartan, NF-κB blocker pyrrolidine dithiocarbamate (PDTC), and specific short peptides from the second extracellular loop of AT1 receptor.
These results suggested that AT1-AAs, via the AT1 receptor, induce expression of proinflammatory cytokines involved in the activation of NF-κB.
AT1-AAs may play a great role in the pathogenesis of the acute coronary syndrome by mediating vascular inflammatory effects involved in the NF-κB pathway.
American Psychological Association (APA)
Li, Weijuan& Li, Zhi& Chen, Yaoqi& Li, Songhai& Lv, Yuanyuan& Zhou, Wenping…[et al.]. 2014. Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1040845
Modern Language Association (MLA)
Li, Weijuan…[et al.]. Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway. Journal of Immunology Research No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1040845
American Medical Association (AMA)
Li, Weijuan& Li, Zhi& Chen, Yaoqi& Li, Songhai& Lv, Yuanyuan& Zhou, Wenping…[et al.]. Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1040845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040845