Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate
Joint Authors
Scott, Andrew E.
Ngugi, Sarah A.
Laws, Thomas R.
Corser, David
Lonsdale, Claire L.
D’Elia, Riccardo V.
Titball, Richard W.
Atkins, Timothy P.
Prior, Joann L.
Williamson, Diane
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei.
It is refractory to antibiotic treatment and there is currently no licensed vaccine.
In this report we detail the construction and protective efficacy of a polysaccharide-protein conjugate composed of B.
pseudomallei lipopolysaccharide and the Hc fragment of tetanus toxin.
Immunisation of mice with the lipopolysaccharide-conjugate led to significantly reduced bacterial burdens in the spleen 48 hours after challenge and afforded significant protection against a lethal challenge with B.
pseudomallei.
The conjugate generated significantly higher levels of antigen-specific IgG1 and IgG2a than in lipopolysaccharide-immunised mice.
Immunisation with the conjugate also demonstrated a bias towards Th1 type responses, evidenced by high levels of IgG2a.
In contrast, immunisation with unconjugated lipopolysaccharide evoked almost no IgG2a demonstrating a bias towards Th2 type responses.
This study demonstrates the effectiveness of this approach in the development of an efficacious and protective vaccine against melioidosis.
American Psychological Association (APA)
Scott, Andrew E.& Ngugi, Sarah A.& Laws, Thomas R.& Corser, David& Lonsdale, Claire L.& D’Elia, Riccardo V.…[et al.]. 2014. Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040858
Modern Language Association (MLA)
Scott, Andrew E.…[et al.]. Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate. Journal of Immunology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040858
American Medical Association (AMA)
Scott, Andrew E.& Ngugi, Sarah A.& Laws, Thomas R.& Corser, David& Lonsdale, Claire L.& D’Elia, Riccardo V.…[et al.]. Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040858
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040858