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Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target
Joint Authors
Wang, Mingxia
Zhu, Shangling
Peng, Weixiang
Li, Qiuxia
Li, Zhaoxia
Luo, Minqi
Feng, Xiaoxue
Lin, Zhuofeng
Huang, Jianlin
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sonic hedgehog (Shh) signaling controls many aspects of human development, regulates cell growth and differentiation in adult tissues, and is activated in a number of malignancies.
Rheumatoid arthritis (RA) is characterized by chronic synovitis and pannus formation associated with activation of fibroblast-like synoviocytes (FLS).
We investigated whether Shh signaling plays a role in the proliferation of FLS in RA.
Expression of Shh signaling related components (Shh, Ptch1, Smo, and Gli1) in RA synovial tissues was examined by immunohistochemistry (IHC) and in FLS by IHC, immunofluorescence (IF), quantitative RT-PCR, and western blotting.
Expression of Shh, Smo, and Gli1 in RA synovial tissue was higher than that in control tissue ( P < 0.05 ).
Cyclopamine (a specific inhibitor of Shh signaling) decreased mRNA expression of Shh, Ptch1, Smo, and Gli1 in cultured RA FLS, Shh, and Smo protein expression, and significantly decreased FLS proliferation.
Flow cytometry analysis suggested that cyclopamine treatment resulted in cell cycle arrest of FLS in G1 phase.
Our data show that Shh signaling is activated in synovium of RA patients in vivo and in cultured FLS form RA patients in vitro, suggesting a role in the proliferation of FLS in RA.
It may therefore be a novel therapeutic target in RA.
American Psychological Association (APA)
Wang, Mingxia& Zhu, Shangling& Peng, Weixiang& Li, Qiuxia& Li, Zhaoxia& Luo, Minqi…[et al.]. 2014. Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040863
Modern Language Association (MLA)
Wang, Mingxia…[et al.]. Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target. Journal of Immunology Research No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1040863
American Medical Association (AMA)
Wang, Mingxia& Zhu, Shangling& Peng, Weixiang& Li, Qiuxia& Li, Zhaoxia& Luo, Minqi…[et al.]. Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1040863
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040863