Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies
Joint Authors
Molesti, Eleonora
Wright, Edward
Rahman, Rafat
Terregino, Calogero
Temperton, Nigel J.
Cattoli, Giovanni
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The increased number of outbreaks of H5 and H7 LPAI and HPAI viruses in poultry has major public and animal health implications.
The continuous rapid evolution of these subtypes and the emergence of new variants influence the ability to undertake effective surveillance.
Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardized influenza serological assays.
We describe a multiplex assay for the study of neutralizing antibodies that are directed against both influenza H5 and H7 HA.
This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera.
Sera obtained from chickens vaccinated with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent H7N1/H5N9 vaccine, or turkeys naturally infected with an H7N3 virus were evaluated in this assay and the results correlated strongly with data obtained by HI assay.
We show that pseudotypes are highly stable under basic cold-chain storage conditions and following multiple rounds of freeze-thaw.
We propose that this robust assay may have practical utility for in-field serosurveillance and vaccine studies in resource-limited regions worldwide.
American Psychological Association (APA)
Molesti, Eleonora& Wright, Edward& Terregino, Calogero& Rahman, Rafat& Cattoli, Giovanni& Temperton, Nigel J.. 2014. Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1040870
Modern Language Association (MLA)
Molesti, Eleonora…[et al.]. Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies. Journal of Immunology Research No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1040870
American Medical Association (AMA)
Molesti, Eleonora& Wright, Edward& Terregino, Calogero& Rahman, Rafat& Cattoli, Giovanni& Temperton, Nigel J.. Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1040870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040870