Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling
Joint Authors
Galván Morales, Miguel Ángel
Cabello Gutiérrez, Carlos
Mejía Nepomuceno, Fidencio
Valle Peralta, Leticia
Valencia Maqueda, Elba
Manjarrez Zavala, María Eugenia
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Human parainfluenza virus type 1 (HPIV-1) is the most common cause of croup in infants.
The aim of this study was to describe molecular mechanisms associated with IL-8 production during HPIV-1 infection and the role of viral replication in MAPK synthesis and activation.
An in vitro model of HPIV-1 infection in the HEp-2 and A549 cell lines was used; a kinetic-based ELISA for IL-8 detection was also used, phosphorylation of the mitogen-activated protein kinases (MAPKs) was identified by Western blot analysis, and specific inhibitors for each kinase were used to identify which MAPK was involved.
Inactivated viruses were used to assess whether viral replication is required for IL-8 production.
Results revealed a gradual increase in IL-8 production at different selected times, when phosphorylation of MAPK was detected.
The secretion of IL-8 in the two cell lines infected with the HPIV-1 is related to the phosphorylation of the MAPK as well as viral replication.
Inhibition of p38 suppressed the secretion of IL-8 in the HEp-2 cells.
No kinase activation was observed when viruses were inactivated.
American Psychological Association (APA)
Galván Morales, Miguel Ángel& Cabello Gutiérrez, Carlos& Mejía Nepomuceno, Fidencio& Valle Peralta, Leticia& Valencia Maqueda, Elba& Manjarrez Zavala, María Eugenia. 2014. Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1040883
Modern Language Association (MLA)
Galván Morales, Miguel Ángel…[et al.]. Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling. Journal of Immunology Research No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1040883
American Medical Association (AMA)
Galván Morales, Miguel Ángel& Cabello Gutiérrez, Carlos& Mejía Nepomuceno, Fidencio& Valle Peralta, Leticia& Valencia Maqueda, Elba& Manjarrez Zavala, María Eugenia. Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1040883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040883