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Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies
Joint Authors
Zvirbliene, Aurelija
Sereika, Vilimas
Sasnauskas, Kestutis
Tamošiūnas, Paulius Lukas
Petraitytė-Burneikienė, Rasa
Akatov, Artiomas
Kundrotas, Gabrielis
Lelešius, Raimundas
Lasickienė, Rita
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets.
The gene coding for the major capsid protein VP2 of PPV was amplified using viral nucleic acid extract from swine serum and inserted into yeast Saccharomyces cerevisiae expression plasmid.
Recombinant PPV VP2 protein was efficiently expressed in yeast and purified using density gradient centrifugation.
Electron microscopy analysis of purified PPV VP2 protein revealed the self-assembly of virus-like particles (VLPs).
Nine monoclonal antibodies (MAbs) against the recombinant PPV VP2 protein were generated.
The specificity of the newly generated MAbs was proven by immunofluorescence analysis of PPV-infected cells.
Indirect IgG ELISA based on the recombinant VLPs for detection of PPV-specific antibodies in swine sera was developed and evaluated.
The sensitivity and specificity of the new assay were found to be 93.4% and 97.4%, respectively.
In conclusion, yeast S.
cerevisiae represents a promising expression system for generating recombinant PPV VP2 protein VLPs of diagnostic relevance.
American Psychological Association (APA)
Tamošiūnas, Paulius Lukas& Petraitytė-Burneikienė, Rasa& Lasickienė, Rita& Akatov, Artiomas& Kundrotas, Gabrielis& Sereika, Vilimas…[et al.]. 2014. Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040905
Modern Language Association (MLA)
Tamošiūnas, Paulius Lukas…[et al.]. Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies. Journal of Immunology Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040905
American Medical Association (AMA)
Tamošiūnas, Paulius Lukas& Petraitytė-Burneikienė, Rasa& Lasickienė, Rita& Akatov, Artiomas& Kundrotas, Gabrielis& Sereika, Vilimas…[et al.]. Generation of Recombinant Porcine Parvovirus Virus-Like Particles in Saccharomyces cerevisiae and Development of Virus-Specific Monoclonal Antibodies. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040905