Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1Th2 Skewness
Joint Authors
Lin, Ling
Huang, Ziyang
Ouyang, Qiufang
Wang, Zhenhua
Chen, Xiaoqing
Ni, Jing-qin
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We investigated the protective role of chloroquine against pristane-induced macrophage activation, oxidative stress, and Th1/Th2 skewness in C57BL/6J mice.
Those mice were treated with pristane alone or combined with chloroquine.
Hematological and biochemical parameters, macrophage phagocytic function, the oxidant/antioxidant index, cytokine for IFN-γ, TNF-α, IL-4, and IL-6, and the isotypes of IgG2a and IgG1 were determined.
And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR.
We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6.
In parallel, a significant increase in lipid peroxidation and a decrease in superoxide dismutase, glutathione, and catalase activity, as well as a skewed Th1/Th2 balance in spleen, were observed.
However, chloroquine supplementation showed a remarkable amelioration of these abnormalities.
Our data indicate that pristane administration induces macrophage activation, oxidative stress, and Th1/Th2 skewness, which can be attenuated by chloroquine.
American Psychological Association (APA)
Ouyang, Qiufang& Huang, Ziyang& Wang, Zhenhua& Chen, Xiaoqing& Ni, Jing-qin& Lin, Ling. 2014. Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1Th2 Skewness. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040921
Modern Language Association (MLA)
Ouyang, Qiufang…[et al.]. Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1Th2 Skewness. Journal of Immunology Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1040921
American Medical Association (AMA)
Ouyang, Qiufang& Huang, Ziyang& Wang, Zhenhua& Chen, Xiaoqing& Ni, Jing-qin& Lin, Ling. Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1Th2 Skewness. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1040921
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040921