Ursodeoxycholic Acid Influences the Expression of p 27 kip1 but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis
Joint Authors
Bogdanos, Dimitrios P.
Kopycińska, Justyna
Haas, Tara
Elias, Elwyn
Milkiewicz, Piotr
Milkiewicz, Małgorzata
Kempinska-Podhorodecka, Agnieszka
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
Enhanced expression of cell cycle inhibitor p 27 kip1 suppresses cell proliferation.
Ursodeoxycholic acid (UDCA) delays progression of primary biliary cirrhosis (PBC) but its effect on p 27 kip1 expression is uncertain.
Aims.
To analyze the expression of p 27 kip1 and its transcription modulator FoxO1 in patients with PBC, and to assess the impact of UDCA on this pathway.
Materials and Methods.
The examined human tissue included explanted livers from patients with cirrhotic PBC ( n = 23 ), primary sclerosing cholangitis (PSC; n = 9 ), alcoholic liver disease (ALD; n = 9 ), and routine liver biopsies from patients with non-cirrhotic PBC ( n = 26 ).
Healthy liver samples served as controls ( n = 19 ).
Livers of FoxO-deficient mice were also studied.
mRNA and protein expressions were analyzed by real-time PCR and Western blot.
Results.
p 27 kip1 expression was increased in cirrhotic and non-cirrhotic PBC.
FoxO1 mRNA levels were increased in PBC (8.5-fold increase versus controls).
FoxO1 protein expression in PBC was comparable to controls, but it was decreased in patients with PSC and ALD (63% and 70% reduction, respectively; both P < 0.05 versus control).
UDCA-treated non-cirrhotic patients with PBC showed decreased expression of p 27 kip1 mRNA.
Conclusion.
PBC progression is characterized by a FoxO1-independent increase of p 27 kip1 expression.
In early PBC, UDCA may enhance liver regeneration via p 27 kip1 -dependent mechanism.
American Psychological Association (APA)
Milkiewicz, Małgorzata& Kopycińska, Justyna& Kempinska-Podhorodecka, Agnieszka& Haas, Tara& Bogdanos, Dimitrios P.& Elias, Elwyn…[et al.]. 2014. Ursodeoxycholic Acid Influences the Expression of p 27 kip1 but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041010
Modern Language Association (MLA)
Milkiewicz, Małgorzata…[et al.]. Ursodeoxycholic Acid Influences the Expression of p 27 kip1 but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis. Journal of Immunology Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1041010
American Medical Association (AMA)
Milkiewicz, Małgorzata& Kopycińska, Justyna& Kempinska-Podhorodecka, Agnieszka& Haas, Tara& Bogdanos, Dimitrios P.& Elias, Elwyn…[et al.]. Ursodeoxycholic Acid Influences the Expression of p 27 kip1 but Not FoxO1 in Patients with Non-Cirrhotic Primary Biliary Cirrhosis. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041010