Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources
Joint Authors
Tredici, Giovanni
Brini, Anna Teresa
Monfrini, Marianna
Donzelli, Elisabetta
Orciani, Monia
Niada, Stefania
Foudah, Dana
Miloso, Mariarosaria
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-05
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence of the MSCs’ predisposition to differentiate toward neural lineages, supporting their use in stem cell-based therapy for neural repair.
In this study we have evaluated, by immunocytochemistry, immunoblotting, and flow cytometry experiments, the expression of neural markers in undifferentiated MSCs from different sources: human adipose stem cells (hASCs), human skin-derived mesenchymal stem cells (hS-MSCs), human periodontal ligament stem cells (hPDLSCs,) and human dental pulp stem cells (hDPSCs).
Our results demonstrate that the neuronal markers βIII-tubulin and NeuN, unlike other evaluated markers, are spontaneously expressed by a very high percentage of undifferentiated hASCs, hS-MSCs, hPDLSCs, and hDPSCs.
Conversely, the neural progenitor marker nestin is expressed only by a high percentage of undifferentiated hPDLSCs and hDPSCs.
Our results suggest that the expression of βIII-tubulin and NeuN could be a common feature of stem cells and not exclusive to neuronal cells.
This could result in a reassessment of the use of βIII-tubulin and NeuN as the only evidence proving neuronal differentiation.
Further studies will be necessary to elucidate the relevance of the spontaneous expression of these markers in stem cells.
American Psychological Association (APA)
Foudah, Dana& Monfrini, Marianna& Donzelli, Elisabetta& Niada, Stefania& Brini, Anna Teresa& Orciani, Monia…[et al.]. 2014. Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1041045
Modern Language Association (MLA)
Foudah, Dana…[et al.]. Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources. Journal of Immunology Research No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1041045
American Medical Association (AMA)
Foudah, Dana& Monfrini, Marianna& Donzelli, Elisabetta& Niada, Stefania& Brini, Anna Teresa& Orciani, Monia…[et al.]. Expression of Neural Markers by Undifferentiated Mesenchymal-Like Stem Cells from Different Sources. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1041045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041045