ApoA-IHDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes
Joint Authors
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Exogenous apolipoprotein A-I (apoA-I) associates with ATP-binding cassette transporter A1 (ABCA1) on the cell surface of astrocytes like various peripheral cells and enhances the translocation of newly synthesized cholesterol from the endoplasmic reticulum/Golgi apparatus (ER/Golgi) to the cytosol.
The cholesterol translocated to the cytosol is incorporated to cytosolic lipid-protein particles (CLPP) together with phospholipids and proteins such as sphingomyelin, phosphatidylcholine, caveolin-1, protein kinase Cα (PK-Cα), and cyclophilin A.
The CLPP are high density lipoproteins- (HDL-)like cytosolic lipid-protein complex with densities of 1.09–1.16 g/mL and diameters of 17-18 nm.
The association of exogenous apoA-I with cellular ABCA1 induces tyrosine phosphorylation, activation, and translocation to the CLPP of ABCA1-associated phospholipase Cγ (PL-Cγ) in rat astrocytes.
Furthermore, PK-Cα is translocated and activated to/in the CLPP through the production of diacylglyceride in the CLPP.
ApoA-I enhances both the association of CLPP with microtubules and the phosphorylation of α-tubulin as a component of microtubules.
The CLPP are dissociated from microtubules after α-tubulin in microtubules is phosphorylated by the CLPP-associated PK-Cα.
The association and dissociation between CLPP and microtubules may participate in the intracellular transport of cholesterol to the plasma membrane.
American Psychological Association (APA)
Ito, Jinichi& Michikawa, Makoto. 2014. ApoA-IHDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes. Journal of Lipids،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041052
Modern Language Association (MLA)
Ito, Jinichi& Michikawa, Makoto. ApoA-IHDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes. Journal of Lipids No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1041052
American Medical Association (AMA)
Ito, Jinichi& Michikawa, Makoto. ApoA-IHDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes. Journal of Lipids. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041052
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041052