Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation
Joint Authors
Suzuki, Masayo
Ohtsuki, Kazuya
Kino, Katsuhito
Kobayashi, Teruhiko
Morikawa, Masayuki
Kobayashi, Takanobu
Miyazawa, Hiroshi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The nucleoside 2,2,4-triamino-5(2H)-oxazolone (Oz) can result from oxidative damage to guanine residues in DNA.
Despite differences among the three polymerases (Pol β, KF exo−, and Pol η) regarding nucleotide incorporation patterns opposite Oz, all three polymerases can incorporate guanine opposite Oz.
Based on ab initio calculations, we proposed a structure for a stable Oz:G base pair.
Here, to assess the stability of each Oz-containing base pair (Oz:G, Oz:A, Oz:C, and Oz:T) upon DNA replication, we determined the efficiency of Pol β-, KF exo−-, or Pol η-catalyzed primer extension beyond each base pair.
With each polymerase, extension beyond Oz:G was more efficient than that beyond Oz:A, Oz:C, or Oz:T.
Moreover, thermal denaturation studies revealed that the T m value for the duplex containing Oz:G was significantly higher than those obtained for duplexes containing Oz:A, Oz:C, or Oz:T.
Therefore, the results from ab initio calculations along with those from DNA replication assays and thermal denaturation experiments supported the conclusion that Oz:G is the most stable of the Oz-containing base pairs.
American Psychological Association (APA)
Suzuki, Masayo& Ohtsuki, Kazuya& Kino, Katsuhito& Kobayashi, Teruhiko& Morikawa, Masayuki& Kobayashi, Takanobu…[et al.]. 2014. Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation. Journal of Nucleic Acids،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041142
Modern Language Association (MLA)
Suzuki, Masayo…[et al.]. Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation. Journal of Nucleic Acids No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1041142
American Medical Association (AMA)
Suzuki, Masayo& Ohtsuki, Kazuya& Kino, Katsuhito& Kobayashi, Teruhiko& Morikawa, Masayuki& Kobayashi, Takanobu…[et al.]. Effects of Stability of Base Pairs Containing an Oxazolone on DNA Elongation. Journal of Nucleic Acids. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041142