Synthesis, Characterization, and DNA Binding Studies of Nanoplumbagin

Abstract EN

The traditional anticancer medicine plumbagin (PLN) was prepared as nanostructured material (nanoplumbagin, NPn1) from its commercial counterparts, simultaneously coencapsulating with cetyltrimethylammonium bromide or cyclodextrin as stabilizers using ultrasonication technique.

Surface morphology of NPn analysed from atomic force microscopy (AFM) indicates that NPn has tunable size between 75 nm and 100 nm with narrow particle size distribution.

Its binding efficiency with herring sperm DNA was studied using spectral and electrochemical techniques and its efficiency was found to be more compared to the commercial microcrystalline plumbagin (PLN).

DNA cleavage was also studied by gel electrophoresis.

The observed results indicate that NPn1 has better solubility in aqueous medium and hence showed better bioavailability compared to its commercial counterparts.