![](/images/graphics-bg.png)
Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery
Joint Authors
Oliveira, Ana Vanessa
Silva, Gabriela A.
Bitoque, Diogo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The low gene transfer efficiency of chitosan-DNA polyplexes is a consequence of their high stability and consequent slow DNA release.
The incorporation of an anionic polymer is believed to loosen chitosan interactions with DNA and thus promote higher transfection efficiencies.
In this work, several formulations of chitosan-DNA polyplexes incorporating hyaluronic acid were prepared and characterized for their gene transfection efficiency on both HEK293 and retinal pigment epithelial cells.
The different polyplex formulations showed morphology, size, and charge compatible with a role in gene delivery.
The incorporation of hyaluronic acid rendered the formulations less stable, as was the goal, but it did not affect the loading and protection of the DNA.
Compared with chitosan alone, the transfection efficiency had a 4-fold improvement, which was attributed to the presence of hyaluronic acid.
Overall, our hybrid chitosan-hyaluronic acid polyplexes showed a significant improvement of the efficiency of chitosan-based nonviral vectors in vitro, suggesting that this strategy can further improve the transfection efficiency of nonviral vectors.
American Psychological Association (APA)
Oliveira, Ana Vanessa& Bitoque, Diogo& Silva, Gabriela A.. 2014. Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery. Journal of Nanomaterials،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041351
Modern Language Association (MLA)
Oliveira, Ana Vanessa…[et al.]. Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery. Journal of Nanomaterials No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1041351
American Medical Association (AMA)
Oliveira, Ana Vanessa& Bitoque, Diogo& Silva, Gabriela A.. Combining Hyaluronic Acid with Chitosan Enhances Gene Delivery. Journal of Nanomaterials. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1041351
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041351