Fabrication of Aligned Carbon NanotubePolycaprolactoneGelatin Nanofibrous Matrices for Schwann Cell Immobilization
Joint Authors
Rau, Lih-Rou
Huang, Chun-Chiang
Hsu, Fu-Yin
Tsai, Shiao-Wen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, we utilized a mandrel rotating collector consisting of two parallel, electrically conductive pieces of tape to fabricate aligned electrospun polycaprolactone/gelatin (PG) and carbon nanotube/polycaprolactone/gelatin (PGC) nanofibrous matrices.
Furthermore, we examined the biological performance of the PGC nanofibrous and film matrices using an in vitro culture of RT4-D6P2T rat Schwann cells.
Using cell adhesion tests, we found that carbon nanotube inhibited Schwann cell attachment on PGC nanofibrous and film matrices.
However, the proliferation rates of Schwann cells were higher when they were immobilized on PGC nanofibrous matrices compared to PGC film matrices.
Using western blot analysis, we found that NRG1 and P0 protein expression levels were higher for cells immobilized on PGC nanofibrous matrices compared to PG nanofibrous matrices.
However, the carbon nanotube inhibited NRG1 and P0 protein expression in cells immobilized on PGC film matrices.
Moreover, the NRG1 and P0 protein expression levels were higher for cells immobilized on PGC nanofibrous matrices compared to PGC film matrices.
We found that the matrix topography and composition influenced Schwann cell behavior.
American Psychological Association (APA)
Tsai, Shiao-Wen& Huang, Chun-Chiang& Rau, Lih-Rou& Hsu, Fu-Yin. 2014. Fabrication of Aligned Carbon NanotubePolycaprolactoneGelatin Nanofibrous Matrices for Schwann Cell Immobilization. Journal of Nanomaterials،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041594
Modern Language Association (MLA)
Tsai, Shiao-Wen…[et al.]. Fabrication of Aligned Carbon NanotubePolycaprolactoneGelatin Nanofibrous Matrices for Schwann Cell Immobilization. Journal of Nanomaterials No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1041594
American Medical Association (AMA)
Tsai, Shiao-Wen& Huang, Chun-Chiang& Rau, Lih-Rou& Hsu, Fu-Yin. Fabrication of Aligned Carbon NanotubePolycaprolactoneGelatin Nanofibrous Matrices for Schwann Cell Immobilization. Journal of Nanomaterials. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1041594
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041594