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Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone-Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA-MB-231 Cell Line
Joint Authors
Rahman, Heshu Sulaiman
Abdullah, Rasedee
Hosseinpour, Mahnaz
Ahmadi, Negin
Chartrand, Max Stanley
Othman, Hemn Hassan
Abdul, Ahmad Bustamam
Yeap, Swee Keong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
This study investigated the anticancer effect of zerumbone (ZER) and zerumbone-loaded nanostructured lipid carrier (ZER-NLC) on the human mammary gland adenocarcinoma (MDA-MB-231) cell line.
The effect of ZER and ZER-NLC on MDA-MB-231 cells was determined via electron and fluorescent microscopy and flow cytometry using the Annexin V, cell cycle, and Tdt-mediated dUTP nick-end labeling assays.
We demonstrated that ZER and ZER-NLC significantly suppressed the proliferation of MDA-MB-231 cells with an IC50 of 5.96 ± 0.13 and 6.01 ± 0.11 μg/mL, respectively.
ZER and ZER-NLC arrested MDA-MB-231 cell cycle at the G2/M phase.
The induction of apoptosis by ZER and ZER-NLC was via the intrinsic pathway through the release of cytochrome c and activation of caspase-3 and caspase-9.
The treatments also caused the downregulation of antiapoptotic Bcl-2, Bcl-xL proteins, and proliferating cell nuclear protein and upregulation of proapoptotic Bax protein.
Therefore, loading of ZER into NLC did not compromise the anticancer effects of ZER on MDA-MB-231 cells.
In conclusion, ZER-NLC, which increased the bioavailability of ZER, is an effective agent in the treatment of cancers.
American Psychological Association (APA)
Hosseinpour, Mahnaz& Abdul, Ahmad Bustamam& Rahman, Heshu Sulaiman& Abdullah, Rasedee& Yeap, Swee Keong& Ahmadi, Negin…[et al.]. 2014. Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone-Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA-MB-231 Cell Line. Journal of Nanomaterials،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1041859
Modern Language Association (MLA)
Hosseinpour, Mahnaz…[et al.]. Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone-Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA-MB-231 Cell Line. Journal of Nanomaterials No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1041859
American Medical Association (AMA)
Hosseinpour, Mahnaz& Abdul, Ahmad Bustamam& Rahman, Heshu Sulaiman& Abdullah, Rasedee& Yeap, Swee Keong& Ahmadi, Negin…[et al.]. Comparison of Apoptotic Inducing Effect of Zerumbone and Zerumbone-Loaded Nanostructured Lipid Carrier on Human Mammary Adenocarcinoma MDA-MB-231 Cell Line. Journal of Nanomaterials. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1041859
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041859