Plasma Treated High-Density Polyethylene (HDPE)‎ Medpor Implant Immobilized with rhBMP-2 for Improving the Bone Regeneration

Abstract EN

We investigate the bone generation capacity of recombinant human bone morphogenetic protein-2 (rhBMP-2) immobilized Medpor surface through acrylic acid plasma-polymerization.

Plasma-polymerization was carried out at a 20 W at an acrylic acid flow rate of 7 sccm for 5 min.

The plasma-polymerized Medpor surface showed hydrophilic properties and possessed a high density of carboxyl groups.

The rhBMP-2 was immobilized with covalently attached carboxyl groups using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide.

Carboxyl groups and rhBMP-2 immobilization on the Medpor surface were identified by Fourier transform infrared spectroscopy.

The activity of Medpor with rhBMP-2 immobilized was examined using an alkaline phosphatase assay on MC3T3-E1 cultured Medpor.

These results showed that the rhBMP-2 immobilized Medpor increased the level of MC3T3-E1 cell differentiation.

These results demonstrated that plasma surface modification has the potential to immobilize rhBMP-2 on polymer implant such as Medpor and can be used for the binding of bioactive nanomolecules in bone tissue engineering.