Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip
Joint Authors
Patthanasettakul, V.
Thepwiwatjit, Nualrahong
Thattiyaphong, Aree
Tuitemwong, Kooranee
Tuitemwong, Pravate
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
This paper describes an application of Rubpy dye-doped silica nanoparticles (RSNPs) as signal reporter in a dot fluorescence immunoassay strip for rapid screening of Vibrio cholera O1 (VCO1).
These nanoparticles have a spherical shape with an average diameter of 45 nm.
They appear luminescent orange when excited with a 312 nm UV lamp.
Based on the sandwich immunoassay principle, a test strip was made of a nitrocellulose membrane dotted with monoclonal antibodies against VCO1 as analyte capture molecules.
After introducing a test sample, followed by polyclonal rabbit anti-VCO1 antibody conjugated RSNPs as detection reporters and one washing step, the presence or absence of the target bacteria could be identified under UV light by naked eyes.
A positive sample would signal a bright orange dot on the strip.
The proposed assay had a detection limit of 4.3 × 10 3 cfu/mL and was successfully applied as a rapid screening test for VCO1 in food samples with high sensitivity, specificity, and accuracy.
American Psychological Association (APA)
Thepwiwatjit, Nualrahong& Thattiyaphong, Aree& Patthanasettakul, V.& Tuitemwong, Kooranee& Tuitemwong, Pravate. 2014. Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip. Journal of Nanomaterials،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1041984
Modern Language Association (MLA)
Thepwiwatjit, Nualrahong…[et al.]. Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip. Journal of Nanomaterials No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1041984
American Medical Association (AMA)
Thepwiwatjit, Nualrahong& Thattiyaphong, Aree& Patthanasettakul, V.& Tuitemwong, Kooranee& Tuitemwong, Pravate. Rubpy Dye-Doped Silica Nanoparticles as Signal Reporter in a Dot Fluorescence Immunoassay Strip. Journal of Nanomaterials. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1041984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1041984