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Oridonin Loaded Solid Lipid Nanoparticles Enhanced Antitumor Activity in MCF-7 Cells
Joint Authors
Wang, Yitao
Wang, Lu
Chen, Ruie
Wang, Yanping
Li, Hui
Wang, Shengpeng
Chen, Mei-Wan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Oridonin (ORI), a famous diterpenoid from Chinese herbal medicine, has drawn rising attention for its remarkable apoptosis and autophagy-inducing activity in human cancer therapy, while clinical application of ORI is limited by its strong hydrophobicity and rapid plasma clearance.
The purpose of this study was to evaluate whether the antitumor activity of ORI could be enhanced by loading into solid lipid nanoparticles (SLNs).
ORI-loaded SLNs were prepared by hot high pressure homogenization with narrow size distribution and good entrapment efficacy.
MTT assay indicated that ORI-loaded SLNs enhanced the inhibition of proliferation against several human cancer cell lines including breast cancer MCF-7 cells, hepatocellular carcinoma HepG 2 cells, and lung carcinoma A549 cells compared with free ORI, while no significant enhancement of toxicity to human mammary epithelial MCF-10A cells was shown.
Meanwhile, flow cytometric analysis demonstrated that ORI-SLNs induced more significant cell cycle arrest at S and decreased cell cycle arrest at G1/G0 phase in MCF-7 cells than bulk ORI solution.
Hoechst 33342 staining and Annexin V/PI assay indicated that apoptotic rates of cells treated with ORI-loaded SLNs were higher compared with free ORI.
In summary, our data indicated that SLNs may be a potential carrier for enhancing the antitumor effect of hydrophobic drug ORI.
American Psychological Association (APA)
Wang, Lu& Wang, Shengpeng& Chen, Ruie& Wang, Yanping& Li, Hui& Wang, Yitao…[et al.]. 2014. Oridonin Loaded Solid Lipid Nanoparticles Enhanced Antitumor Activity in MCF-7 Cells. Journal of Nanomaterials،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1042020
Modern Language Association (MLA)
Wang, Lu…[et al.]. Oridonin Loaded Solid Lipid Nanoparticles Enhanced Antitumor Activity in MCF-7 Cells. Journal of Nanomaterials No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1042020
American Medical Association (AMA)
Wang, Lu& Wang, Shengpeng& Chen, Ruie& Wang, Yanping& Li, Hui& Wang, Yitao…[et al.]. Oridonin Loaded Solid Lipid Nanoparticles Enhanced Antitumor Activity in MCF-7 Cells. Journal of Nanomaterials. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1042020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042020