Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs
Joint Authors
Alvizo Báez, Cynthia Aracely
Luna Cruz, Itza Eloisa
Rodríguez Padilla, Maria Cristina
Alcocer González, Juan Manuel
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-12
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
One of the major problems of gene therapy is the efficient, specific, and targeted delivery as well as the safety of the materials used in such systems.
The specific targeted delivery of genes to the lung offers the possibility to treat a variety of specific diseases.
We developed chitosan nanoparticles with the plasmid pCEM-Luc, which contains a promoter activated by magnetic field.
Nanoparticles of 200–250 nm obtained by ionic gelation with a 99% retention rate were transfected in B16F10 cells and in vivo in the lungs of Balb/c mice by intratracheal administration.
We observed that an external magnetic field increased the expression of the luciferase reporter gene in B16F10 cells transfected with magnetic nanoparticles and in homogenized lungs of mice which determined differences in levels of expression between different regions of the lungs (apical or distal and left or right).
The highest levels of luciferase activity were observed in the apical left region.
The magnetic nanoparticles prove an efficient delivery system to in vitro transfection of cells and lung tissue.
American Psychological Association (APA)
Alvizo Báez, Cynthia Aracely& Luna Cruz, Itza Eloisa& Rodríguez Padilla, Maria Cristina& Alcocer González, Juan Manuel. 2014. Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs. Journal of Nanotechnology،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1042193
Modern Language Association (MLA)
Alvizo Báez, Cynthia Aracely…[et al.]. Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs. Journal of Nanotechnology No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1042193
American Medical Association (AMA)
Alvizo Báez, Cynthia Aracely& Luna Cruz, Itza Eloisa& Rodríguez Padilla, Maria Cristina& Alcocer González, Juan Manuel. Magnetic Nanoparticles of Chitosan for Targeted Delivery System of Plasmids to the Lungs. Journal of Nanotechnology. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1042193
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042193