Intravitreal and Subconjunctival Melphalan for Retinoblastoma in Transgenic Mice
Joint Authors
Murray, Timothy G.
Shah, Nisha V.
Pham, D. G.
Decatur, C.
Hernandez, E.
Shah, Nikesh N.
Cavalcante, M.
Houston, S. K.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
To measure the chemotherapeutic effects of focal melphalan (intravitreal and subconjunctival) on tumor burden, hypoxia, and vasculature in LHBETATAG murine retinoblastoma model.
Methods.
LHBETATAG transgenic mice were treated with a single 1 mcg intravitreal injection of melphalan, 100 mcg subconjunctival injection, or semiweekly 10 mcg subconjunctival injections for 3 weeks.
At 1 or 3 weeks, eyes were enucleated, serially sectioned, and processed with haematoxylin and eosin (H&E) for tumor burden measurements and probed with immunofluorescence to analyze tumor hypoxia and vasculature.
Results.
Focal melphalan significantly reduced retinal tumor size (P < 0.02) when given intravitreally or subconjunctivally.
Eyes treated with a one-time intravitreal injection of 1 mcg melphalan had significantly smaller tumors at both 1 week (P = 0.017) and at 3 weeks after injection (P = 0.005).
Intratumoral hypoxia showed a significant decline in hypoxia at 1 week following intravitreal injection and after maximum dosage of subconjunctival melphalan.
Total vasculature was not significantly affected following intravitreal administration.
Conclusion.
Focal delivery of melphalan via intravitreal or subconjunctival injection has a significant effect on reducing tumor burden, hypoxia, and vasculature, in the treatment of murine retinoblastoma tumors.
American Psychological Association (APA)
Shah, Nisha V.& Pham, D. G.& Murray, Timothy G.& Decatur, C.& Hernandez, E.& Shah, Nikesh N.…[et al.]. 2014. Intravitreal and Subconjunctival Melphalan for Retinoblastoma in Transgenic Mice. Journal of Ophthalmology،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1042631
Modern Language Association (MLA)
Shah, Nisha V.…[et al.]. Intravitreal and Subconjunctival Melphalan for Retinoblastoma in Transgenic Mice. Journal of Ophthalmology No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1042631
American Medical Association (AMA)
Shah, Nisha V.& Pham, D. G.& Murray, Timothy G.& Decatur, C.& Hernandez, E.& Shah, Nikesh N.…[et al.]. Intravitreal and Subconjunctival Melphalan for Retinoblastoma in Transgenic Mice. Journal of Ophthalmology. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1042631
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042631