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Characterization of the Merkel Cell Carcinoma miRNome
Joint Authors
Ning, Matthew S.
Kim, Annette S.
Prasad, Nripesh
Levy, Shawn E.
Zhang, Huiqiu
Andl, Thomas
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
MicroRNAs have been implicated in various skin cancers, including melanoma, squamous cell carcinoma, and basal cell carcinoma; however, the expression of microRNAs and their role in Merkel cell carcinoma (MCC) have yet to be explored in depth.
To identify microRNAs specific to MCC (MCC-miRs), next-generation sequencing (NGS) of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin.
Comparison of the profiles identified several microRNAs upregulated and downregulated in MCC.
For validation, their expression was measured via qRT-PCR in a larger group of MCC and in a comparison group of non-MCC cutaneous tumors and normal skin.
Eight microRNAs were upregulated in MCC: miR-502-3p, miR-9, miR-7, miR-340, miR-182, miR-190b, miR-873, and miR-183.
Three microRNAs were downregulated: miR-3170, miR-125b, and miR-374c.
Many of these MCC-miRs, the miR-183/182/96a cistron in particular, have connections to tumorigenic pathways implicated in MCC pathogenesis.
In situ hybridization confirmed that the highly expressed MCC-miR, miR-182, is localized within tumor cells.
Furthermore, NGS and qRT-PCR reveal that several of these MCC-miRs are highly expressed in the patient-derived MCC cell line, MS-1.
These data indicate that we have identified a set of MCC-miRs with important implications for MCC research.
American Psychological Association (APA)
Ning, Matthew S.& Kim, Annette S.& Prasad, Nripesh& Levy, Shawn E.& Zhang, Huiqiu& Andl, Thomas. 2014. Characterization of the Merkel Cell Carcinoma miRNome. Journal of Skin Cancer،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043024
Modern Language Association (MLA)
Ning, Matthew S.…[et al.]. Characterization of the Merkel Cell Carcinoma miRNome. Journal of Skin Cancer No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043024
American Medical Association (AMA)
Ning, Matthew S.& Kim, Annette S.& Prasad, Nripesh& Levy, Shawn E.& Zhang, Huiqiu& Andl, Thomas. Characterization of the Merkel Cell Carcinoma miRNome. Journal of Skin Cancer. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043024