Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy
Joint Authors
Ma, Gang
Zhang, Hong
Guo, Jianhua
Zeng, Xiaodan
Hu, Xiaoqian
Hao, Wenying
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-06
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The inhibitory effect of rifampicin on the amyloid formation of hen egg white lysozyme was assessed with both Thioflavin T (ThT) fluorescence assay and Fourier transform infrared (FTIR) difference spectroscopy.
We reveal that ThT fluorescence assay gives a false positive result due to rifampicin interference, while FTIR difference spectroscopy provides a reliable assessment.
With FTIR, we show that rifampicin only has marginally inhibitory effect.
We then propose that FTIR difference spectroscopy can potentially be a convenient method for inhibitor screening in amyloid study.
American Psychological Association (APA)
Ma, Gang& Zhang, Hong& Guo, Jianhua& Zeng, Xiaodan& Hu, Xiaoqian& Hao, Wenying. 2014. Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy. Journal of Spectroscopy،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043086
Modern Language Association (MLA)
Ma, Gang…[et al.]. Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy. Journal of Spectroscopy No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1043086
American Medical Association (AMA)
Ma, Gang& Zhang, Hong& Guo, Jianhua& Zeng, Xiaodan& Hu, Xiaoqian& Hao, Wenying. Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy. Journal of Spectroscopy. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043086
