Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis
Joint Authors
Chikhirzhina, Elena
Starkova, Tatyana
Polyanichko, Alexander M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Interaction of HMGB1 nonhistone chromosomal protein with DNA was studied using circular dichroism spectroscopy and thermal denaturation of DNA.
Melting DNA in the complex was shown to be a biphasic process.
The characteristic melting temperatures of unbound DNA and the DNA bound to HMGB1 in 0.25 mM EDTA solutions were found to be TmI=44.0±0.5°C and TmII=62.0±1°C, respectively.
It was shown that the binding of the HMGB1 molecule affects the melting of the DNA region approximately 30 b.p.
long.
American Psychological Association (APA)
Chikhirzhina, Elena& Starkova, Tatyana& Polyanichko, Alexander M.. 2014. Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis. Journal of Spectroscopy،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043091
Modern Language Association (MLA)
Chikhirzhina, Elena…[et al.]. Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis. Journal of Spectroscopy No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1043091
American Medical Association (AMA)
Chikhirzhina, Elena& Starkova, Tatyana& Polyanichko, Alexander M.. Interaction between Chromosomal Protein HMGB1 and DNA Studied by DNA-Melting Analysis. Journal of Spectroscopy. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043091
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043091
