Proliferation and TH1TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro
Joint Authors
Venkatesan, Vijayalakshmi
Mandarapu, Rajesh
Ajumeera, Rajanna
Prakhya, Balakrishna Murthy
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In recent times, human cell-based assays are gaining attention in assessments of immunomodulatory effects of chemicals.
In the study here, the possible effects of cypermethrin and mancozeb on lymphocyte proliferation and proinflammatory (tumor necrosis factor (TNF-) α) and immunoregulatory cytokine (interferon- (IFN-) γ, interleukins (IL) 2, 4, 6, and 10) formation in vitro were investigated.
Human peripheral blood mononuclear cells (PBMC) were isolated and exposed for 6 hr to noncytotoxic doses (0.45–30 µM) of cypermethrin or mancozeb in the presence of activating rat S9 fraction.
Cultures were then further incubated for 48 or 72 hr in fresh medium containing phytohemagglutinin (10 µg/mL) to assess, respectively, effects on cell proliferation (BrdU-ELISA method) and cytokine formation (flow cytometric bead immunoassays).
Mancozeb induced dose-dependent increases in lymphocyte proliferation, inhibition of production of TNFα and the TH2 cytokines IL-6 and IL-10, and an increase in IFNγ (TH1 cytokine) production (at least 2-fold compared to control); mancozeb also induced inhibition of IL-4 (TH2) and stimulated IL-2 (TH1) production, albeit only in dose-related manners for each.
In contrast, cypermethrin exposure did not cause significant effects on proliferation or cytokine profiles.
Further studies are needed to better understand the functional significance of our in vitro findings.
American Psychological Association (APA)
Mandarapu, Rajesh& Ajumeera, Rajanna& Venkatesan, Vijayalakshmi& Prakhya, Balakrishna Murthy. 2014. Proliferation and TH1TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043185
Modern Language Association (MLA)
Mandarapu, Rajesh…[et al.]. Proliferation and TH1TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro. Journal of Toxicology No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043185
American Medical Association (AMA)
Mandarapu, Rajesh& Ajumeera, Rajanna& Venkatesan, Vijayalakshmi& Prakhya, Balakrishna Murthy. Proliferation and TH1TH2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and Mancozeb In Vitro. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043185