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Botulinum Toxin Suppression of CNS Network Activity In Vitro
Joint Authors
Pancrazio, Joseph J.
Gopal, Kamakshi
Keefer, Edward W.
Gross, Guenter W.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The botulinum toxins are potent agents which disrupt synaptic transmission.
While the standard method for BoNT detection and quantification is based on the mouse lethality assay, we have examined whether alterations in cultured neuronal network activity can be used to detect the functional effects of BoNT.
Murine spinal cord and frontal cortex networks cultured on substrate integrated microelectrode arrays allowed monitoring of spontaneous spike and burst activity with exposure to BoNT serotype A (BoNT-A).
Exposure to BoNT-A inhibited spike activity in cultured neuronal networks where, after a delay due to toxin internalization, the rate of activity loss depended on toxin concentration.
Over a 30 hr exposure to BoNT-A, the minimum concentration detected was 2 ng/mL, a level consistent with mouse lethality studies.
A small proportion of spinal cord networks, but not frontal cortex networks, showed a transient increase in spike and burst activity with exposure to BoNT-A, an effect likely due to preferential inhibition of inhibitory synapses expressed in this tissue.
Lastly, prior exposure to human-derived antisera containing neutralizing antibodies prevented BoNT-A induced inhibition of network spike activity.
These observations suggest that the extracellular recording from cultured neuronal networks can be used to detect and quantify functional BoNT effects.
American Psychological Association (APA)
Pancrazio, Joseph J.& Gopal, Kamakshi& Keefer, Edward W.& Gross, Guenter W.. 2014. Botulinum Toxin Suppression of CNS Network Activity In Vitro. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043209
Modern Language Association (MLA)
Pancrazio, Joseph J.…[et al.]. Botulinum Toxin Suppression of CNS Network Activity In Vitro. Journal of Toxicology No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043209
American Medical Association (AMA)
Pancrazio, Joseph J.& Gopal, Kamakshi& Keefer, Edward W.& Gross, Guenter W.. Botulinum Toxin Suppression of CNS Network Activity In Vitro. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043209