Modeling In Vitro Cellular Responses to Silver Nanoparticles

Joint Authors

Georgopoulos, Panos G.
Chung, Kian Fan
Schwander, Stephan
Mukherjee, Dwaipayan
Royce, Steven G.
Sarkar, Srijata
Thorley, Andrew
Ryan, Mary P.
Porter, Alexandra E.
Tetley, Teresa D.
Zhang, Junfeng

Source

Journal of Toxicology

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-10-09

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Medicine

Abstract EN

Engineered nanoparticles (NPs) have been widely demonstrated to induce toxic effects to various cell types.

In vitro cell exposure systems have high potential for reliable, high throughput screening of nanoparticle toxicity, allowing focusing on particular pathways while excluding unwanted effects due to other cells or tissue dosimetry.

The work presented here involves a detailed biologically based computational model of cellular interactions with NPs; it utilizes measurements performed in human cell culture systems in vitro, to develop a mechanistic mathematical model that can support analysis and prediction of in vivo effects of NPs.

The model considers basic cellular mechanisms including proliferation, apoptosis, and production of cytokines in response to NPs.

This new model is implemented for macrophages and parameterized using in vitro measurements of changes in cellular viability and mRNA levels of cytokines: TNF, IL-1b, IL-6, IL-8, and IL-10.

The model includes in vitro cellular dosimetry due to nanoparticle transport and transformation.

Furthermore, the model developed here optimizes the essential cellular parameters based on in vitro measurements, and provides a “stepping stone” for the development of more advanced in vivo models that will incorporate additional cellular and NP interactions.

American Psychological Association (APA)

Mukherjee, Dwaipayan& Royce, Steven G.& Sarkar, Srijata& Thorley, Andrew& Schwander, Stephan& Ryan, Mary P.…[et al.]. 2014. Modeling In Vitro Cellular Responses to Silver Nanoparticles. Journal of Toxicology،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043214

Modern Language Association (MLA)

Mukherjee, Dwaipayan…[et al.]. Modeling In Vitro Cellular Responses to Silver Nanoparticles. Journal of Toxicology No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1043214

American Medical Association (AMA)

Mukherjee, Dwaipayan& Royce, Steven G.& Sarkar, Srijata& Thorley, Andrew& Schwander, Stephan& Ryan, Mary P.…[et al.]. Modeling In Vitro Cellular Responses to Silver Nanoparticles. Journal of Toxicology. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1043214

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1043214