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The Beneficial Effects of Valproic Acid in Thyroid Cancer Are Mediated through Promoting Redifferentiation and Reducing Stemness Level: An In Vitro Study
Joint Authors
Haghpanah, Vahid
Malehmir, Mohsen
Ahmadian, Shahin
Ghavamzadeh, Ardeshir
Ghaffari, Seyed H.
Alimoghaddam, Kamran
Heshmat, Ramin
Adabi, Khadijeh
Larijani, Bagher
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Valproic acid (VPA) has been identified as a histone deacetylase inhibitor, inducing differentiation in transformed cells.
However, no study has shown the effect of VPA in the redifferentiation induction and stemness of anaplastic thyroid.
The main objective of this study was to evaluate the efficacy of VPA as a differentiation therapy agent in human thyroid cancer based on its effect on stemness and differentiation process.
Indications for differentiation of 8305C and B-CPAP cell lines following VPA treatment were obtained by analyzing cell proliferation rate, morphological changes, adherent-dependent colony formation, and Hoechst 33342 staining.
The expressions of stemness, differentiation, and aggressiveness specific marker genes were measured by quantitative RT-PCR.
VPA treatment effectively showed growth inhibition in both cell lines.
The high nuclear-cytoplasmic (N : C) ratio of 8305C cells markedly decreased and treated cells became more epithelial-like.
Treated cells showed stronger Hoechst 33342 fluorescence compared with control cells.
The hTERT and OCT-4 reduction was paralleled with adherent-dependent colony formation decrement in both cell lines.
VPA effectively induced NIS and TTF-1 in anaplastic cells, it whereas showed no clear pattern in papillary cell line.
VPA treatment also resulted in the reduction of MMP-2 and MMP-9.
These finding suggest that VPA could redifferentiate the anaplastic thyroid cancer cells.
American Psychological Association (APA)
Haghpanah, Vahid& Malehmir, Mohsen& Larijani, Bagher& Ahmadian, Shahin& Alimoghaddam, Kamran& Heshmat, Ramin…[et al.]. 2014. The Beneficial Effects of Valproic Acid in Thyroid Cancer Are Mediated through Promoting Redifferentiation and Reducing Stemness Level: An In Vitro Study. Journal of Thyroid Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043257
Modern Language Association (MLA)
Haghpanah, Vahid…[et al.]. The Beneficial Effects of Valproic Acid in Thyroid Cancer Are Mediated through Promoting Redifferentiation and Reducing Stemness Level: An In Vitro Study. Journal of Thyroid Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043257
American Medical Association (AMA)
Haghpanah, Vahid& Malehmir, Mohsen& Larijani, Bagher& Ahmadian, Shahin& Alimoghaddam, Kamran& Heshmat, Ramin…[et al.]. The Beneficial Effects of Valproic Acid in Thyroid Cancer Are Mediated through Promoting Redifferentiation and Reducing Stemness Level: An In Vitro Study. Journal of Thyroid Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043257