Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1
Joint Authors
Oliveira, Suellen D’arc Santos
Nanini, Hayandra Ferreira
Savio, Luiz Eduardo Baggio
Waghabi, Mariana Caldas
Silva, Claudia Lucia Martins
Coutinho-Silva, Robson
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Schistosomiasis is a chronic inflammatory disease whose macrophages are involved in immunopathology modulation.
Although P2X7 receptor signaling plays an important role in inflammatory responses mediated by macrophages, no reports have examined the role of P2X7 receptors in macrophage function during schistosomiasis.
Thus, we evaluated P2X7 receptor function in peritoneal macrophages during schistosomiasis using an ATP-induced permeabilization assay and measurements of the intracellular Ca2+ concentration.
ATP treatment induced significantly less permeabilization in macrophages from S.
mansoni-infected mice than in control cells from uninfected animals.
Furthermore, P2X7-mediated increases in intracellular Ca2+ levels were also reduced in macrophages from infected mice.
TGF-β1 levels were increased in the peritoneal cavity of infected animals, and pretreatment of control macrophages with TGF-β1 reduced ATP-induced permeabilization, mimicking the effect of S.
mansoni infection.
Western blot and qRT-PCR data showed no difference in P2X7 protein and mRNA between uninfected, infected, and TGF-β1-treated groups.
However, immunofluorescence analysis revealed reduced cell surface localization of P2X7 receptors in macrophages from infected and TGF-β1-treated mice compared to controls.
Therefore, our data suggest that schistosomiasis reduces peritoneal macrophage P2X7 receptor signaling.
This effect is likely due to the fact that infected mice have increased levels of TGF-β1, which reduces P2X7 receptor cell surface expression.
American Psychological Association (APA)
Oliveira, Suellen D’arc Santos& Nanini, Hayandra Ferreira& Savio, Luiz Eduardo Baggio& Waghabi, Mariana Caldas& Silva, Claudia Lucia Martins& Coutinho-Silva, Robson. 2014. Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043331
Modern Language Association (MLA)
Oliveira, Suellen D’arc Santos…[et al.]. Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043331
American Medical Association (AMA)
Oliveira, Suellen D’arc Santos& Nanini, Hayandra Ferreira& Savio, Luiz Eduardo Baggio& Waghabi, Mariana Caldas& Silva, Claudia Lucia Martins& Coutinho-Silva, Robson. Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043331
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043331