Interaction with Mesenchymal Stem Cells Provokes Natural Killer Cells for Enhanced IL-12IL-18-Induced Interferon-Gamma Secretion
Joint Authors
Flohé, Stefanie B.
Jäger, Marcus
Thomas, Heike
Mauel, Katharina
Brandau, Sven
Lask, Sara
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tissue injury induces an inflammatory response accompanied by the recruitment of immune cells and of mesenchymal stem cells (MSC) that contribute to tissue regeneration.
After stimulation with interleukin- (IL-) 12 and IL-18 natural killer (NK) cells secrete the proinflammatory cytokine interferon- (IFN-) γ.
IFN-γ plays a crucial role in the defense against infections and modulates tissue regeneration.
In consideration of close proximity of NK cells and MSC at the site of injury we investigated if MSC could influence the ability of NK-cells to produce IFN-γ.
Coculture experiments were performed with bone marrow-derived human MSC and human NK cells.
MSC enhanced the ability of IL-12/IL-18-stimulated NK cells to secrete IFN-γ in a dose-dependent manner.
This activation of NK cells was dependent on cell-cell contact as well as on soluble factors.
The increased IFN-γ secretion from NK cells after contact with MSC correlated with an increased level of intracellular IFN-γ.
Alterations in the IL-12 signaling pathway including an increased expression of the IL-12 β 1 receptor subunit and an increased phosphorylation of signal transducer and activator of transcription 4 (STAT4) could be observed.
In conclusion, MSC enhance the IFN-γ release from NK cells which might improve the defense against infections at the site of injury but additionally might affect tissue regeneration.
American Psychological Association (APA)
Thomas, Heike& Jäger, Marcus& Mauel, Katharina& Brandau, Sven& Lask, Sara& Flohé, Stefanie B.. 2014. Interaction with Mesenchymal Stem Cells Provokes Natural Killer Cells for Enhanced IL-12IL-18-Induced Interferon-Gamma Secretion. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043341
Modern Language Association (MLA)
Thomas, Heike…[et al.]. Interaction with Mesenchymal Stem Cells Provokes Natural Killer Cells for Enhanced IL-12IL-18-Induced Interferon-Gamma Secretion. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043341
American Medical Association (AMA)
Thomas, Heike& Jäger, Marcus& Mauel, Katharina& Brandau, Sven& Lask, Sara& Flohé, Stefanie B.. Interaction with Mesenchymal Stem Cells Provokes Natural Killer Cells for Enhanced IL-12IL-18-Induced Interferon-Gamma Secretion. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043341