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Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids
Joint Authors
Szóstek, Anna Z.
Galvão, Antonio M.
Hojo, Takuo
Okuda, Kiyoshi
Skarzynski, Dariusz J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro.
In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT.
In Experiment 2, cells were exposed to progesterone (P4; 10−7 M); 17-β estradiol (E2; 10−9 M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h.
The oxytocin (OT; 10−7 M) was used as a positive control.
In Experiment 3, cells were exposed to P4 (10−7 M), E2 (10−9 M) or P4+E2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR.
Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method.
Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells.
This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy.
American Psychological Association (APA)
Szóstek, Anna Z.& Galvão, Antonio M.& Hojo, Takuo& Okuda, Kiyoshi& Skarzynski, Dariusz J.. 2014. Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043378
Modern Language Association (MLA)
Szóstek, Anna Z.…[et al.]. Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043378
American Medical Association (AMA)
Szóstek, Anna Z.& Galvão, Antonio M.& Hojo, Takuo& Okuda, Kiyoshi& Skarzynski, Dariusz J.. Interleukins Affect Equine Endometrial Cell Function: Modulatory Action of Ovarian Steroids. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043378
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043378