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In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure
Joint Authors
Secchiero, Paola
Rizzo, P.
Tisato, Veronica
Voltan, Rebecca
Fucili, Alessandro
Pannella, Micaela
Ferrari, Roberto
Marci, Roberto
Zauli, Giorgio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-23
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Although myocardial angiogenesis is thought to play an important role in heart failure (HF), the involvement of circulating proinflammatory and proangiogenic cytokines in the pathogenesis and/or prognosis of HF has not been deeply investigated.
By using a highly standardized proliferation assay with human endothelial cells, we first demonstrated that sera from older (mean age 52±7.6 years; n=46) healthy donors promoted endothelial cell proliferation to a significantly higher extent compared to sera obtained from younger healthy donors (mean age 29±8.6 years; n=20).
The promotion of endothelial cell proliferation was accompanied by high serum levels of several proangiogenic cytokines.
When we assessed endothelial cell proliferation in response to HF patients’ sera, we observed that a subset of sera (n=11) promoted cell proliferation to a significantly lesser extent compared to the majority of sera (n=18).
Also, in this case, the difference between the patient groups in the ability to induce endothelial cell proliferation correlated to significant (P<0.05) differences in serum proangiogenic cytokine levels.
Unexpectedly, HF patients associated to the highest endothelial proliferation index showed the worst prognosis as evaluated in terms of subsequent cardiovascular events in the follow-up, suggesting that high levels of circulating proangiogenic cytokines might be related to a worse prognosis.
American Psychological Association (APA)
Voltan, Rebecca& Zauli, Giorgio& Rizzo, P.& Fucili, Alessandro& Pannella, Micaela& Marci, Roberto…[et al.]. 2014. In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043406
Modern Language Association (MLA)
Voltan, Rebecca…[et al.]. In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043406
American Medical Association (AMA)
Voltan, Rebecca& Zauli, Giorgio& Rizzo, P.& Fucili, Alessandro& Pannella, Micaela& Marci, Roberto…[et al.]. In Vitro Endothelial Cell Proliferation Assay Reveals Distinct Levels of Proangiogenic Cytokines Characterizing Sera of Healthy Subjects and of Patients with Heart Failure. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043406