MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells
Joint Authors
Stumpfova, Zuzana
Hezova, Renata
Meli, Albano Carlo
Slaby, Ondrej
Michalek, Jaroslav
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Dendritic cells (DCs) belong to the immune system and are particularly studied for their potential to direct either an activated or tolerogenic immune response.
The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation are being increasingly investigated.
This study’s aim is to evaluate the miRNAs’ expression changes in prepared human immature (iDCs), activated (aDCs), and tolerogenic dendritic cells (tDCs).
The dendritic cells were prepared using GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-γ (aDC) or IL-10 and TGF-β (tDC).
Surface markers, cytokine profiles, and miRNA profiles were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation.
We identified 4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC.
We found 5 miRNAs (miR-10a, miR-203, miR-210, miR-30a, and miR-449b) upregulated and 3 miRNAs downregulated (miR-134, miR-145, and miR-149) in both tDC and aDC.
These results indicate that miRNAs are specifically modulated in human DC types.
This work may contribute to identifying specific modulating miRNAs for aDC and tDC, which could in the future serve as therapeutic targets in the treatment of cancer and autoimmune diseases.
American Psychological Association (APA)
Stumpfova, Zuzana& Hezova, Renata& Meli, Albano Carlo& Slaby, Ondrej& Michalek, Jaroslav. 2014. MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043408
Modern Language Association (MLA)
Stumpfova, Zuzana…[et al.]. MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043408
American Medical Association (AMA)
Stumpfova, Zuzana& Hezova, Renata& Meli, Albano Carlo& Slaby, Ondrej& Michalek, Jaroslav. MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043408