Anti-HMGB1 Neutralizing Antibody Ameliorates Neutrophilic Airway Inflammation by Suppressing Dendritic Cell-Mediated Th17 Polarization
Joint Authors
Song, Yong
Zhang, Fang
Huang, Gang
Hu, Bo
Fang, Li-Ping
Cao, E-Hong
Xin, Xiao-Feng
Shi, Yi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We demonstrate that high mobility group box 1 protein (HMGB1) directs Th17 skewing by regulating dendritic cell (DC) function.
First, our in vitro studies reveal that recombinant HMGB1 (rHMGB1) activates myeloid DCs to produce IL-23 in vitro, and rHMGB1-activated DCs prime naïve lymphocytes to produce the Th17 cytokine IL-17A.
Second, we demonstrate that anti-HMGB1 neutralizing antibody attenuates HMGB1 expression, neutrophilic inflammation, airway hyperresponsiveness, and Th17-related cytokine secretion in vivo by using a murine model of neutrophilic asthma induced by ovalbumin (OVA) plus lipopolysaccharide (LPS).
Furthermore, anti-HMGB1 neutralizing antibody decreases the number of Th17 cells in lung cells and suppresses the production of IL-23 by lung CD11C+ APCs.
Finally, we show that intranasal adoptive transfer of rHMGB1-activated DCs was sufficient to restore lung neutrophilic inflammation and the Th17 response in a DC-driven model of asthma, whereas the transfer of rHMGB1 plus anti-HMGB1-treated mDCs significantly reduced these inflammation phenotypes.
These data suggest, for the first time, that HMGB1 drives the DC-polarized Th17-type response in allergic lung inflammation and that blocking HMGB1 may benefit the attenuation of neutrophilic airway inflammation in asthma.
American Psychological Association (APA)
Zhang, Fang& Huang, Gang& Hu, Bo& Fang, Li-Ping& Cao, E-Hong& Xin, Xiao-Feng…[et al.]. 2014. Anti-HMGB1 Neutralizing Antibody Ameliorates Neutrophilic Airway Inflammation by Suppressing Dendritic Cell-Mediated Th17 Polarization. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043459
Modern Language Association (MLA)
Zhang, Fang…[et al.]. Anti-HMGB1 Neutralizing Antibody Ameliorates Neutrophilic Airway Inflammation by Suppressing Dendritic Cell-Mediated Th17 Polarization. Mediators of Inflammation No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1043459
American Medical Association (AMA)
Zhang, Fang& Huang, Gang& Hu, Bo& Fang, Li-Ping& Cao, E-Hong& Xin, Xiao-Feng…[et al.]. Anti-HMGB1 Neutralizing Antibody Ameliorates Neutrophilic Airway Inflammation by Suppressing Dendritic Cell-Mediated Th17 Polarization. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1043459
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043459