Interaction of Apoptotic Cells with Macrophages Upregulates COX-2PGE 2 and HGF Expression via a Positive Feedback Loop
Joint Authors
Byun, Ji Yeon
Youn, Young-So
Lee, Ye-JI
Choi, Youn-Hee
Woo, So-Yeon
Kang, Jihee Lee
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-15
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Recognition of apoptotic cells by macrophages is crucial for resolution of inflammation, immune tolerance, and tissue repair.
Cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) and hepatocyte growth factor (HGF) play important roles in the tissue repair process.
We investigated the characteristics of macrophage COX-2 and PGE2 expression mediated by apoptotic cells and then determined how macrophages exposed to apoptotic cells in vitro and in vivo orchestrate the interaction between COX-2/PGE2 and HGF signaling pathways.
Exposure of RAW 264.7 cells and primary peritoneal macrophages to apoptotic cells resulted in induction of COX-2 and PGE2.
The COX-2 inhibitor NS-398 suppressed apoptotic cell-induced PGE2 production.
Both NS-398 and COX-2-siRNA, as well as the PGE2 receptor EP2 antagonist, blocked HGF expression in response to apoptotic cells.
In addition, the HGF receptor antagonist suppressed increases in COX-2 and PGE2 induction.
The in vivo relevance of the interaction between the COX-2/PGE2 and HGF pathways through a positive feedback loop was shown in cultured alveolar macrophages following in vivo exposure of bleomycin-stimulated lungs to apoptotic cells.
Our results demonstrate that upregulation of the COX-2/PGE2 and HGF in macrophages following exposure to apoptotic cells represents a mechanism for mediating the anti-inflammatory and antifibrotic consequences of apoptotic cell recognition.
American Psychological Association (APA)
Byun, Ji Yeon& Youn, Young-So& Lee, Ye-JI& Choi, Youn-Hee& Woo, So-Yeon& Kang, Jihee Lee. 2014. Interaction of Apoptotic Cells with Macrophages Upregulates COX-2PGE 2 and HGF Expression via a Positive Feedback Loop. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1043565
Modern Language Association (MLA)
Byun, Ji Yeon…[et al.]. Interaction of Apoptotic Cells with Macrophages Upregulates COX-2PGE 2 and HGF Expression via a Positive Feedback Loop. Mediators of Inflammation No. 2014 (2014), pp.1-17.
https://search.emarefa.net/detail/BIM-1043565
American Medical Association (AMA)
Byun, Ji Yeon& Youn, Young-So& Lee, Ye-JI& Choi, Youn-Hee& Woo, So-Yeon& Kang, Jihee Lee. Interaction of Apoptotic Cells with Macrophages Upregulates COX-2PGE 2 and HGF Expression via a Positive Feedback Loop. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-17.
https://search.emarefa.net/detail/BIM-1043565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043565